PhD in Physical Chemistry, 2012
University of New Hampshire
BS in Biomathematics, 2007
Rutgers University
BS in Biotechnology, 2007
Rutgers University
7 Years Industry, 4 Years Academia
19
Over 200
Programs in the Genomic Medicine Unit include viral vectors (e.g., AAV), non-viral vectors (e.g., lipid nanoparticle (LNP)), and cell therapies. My core responsibilities are to support these programs by building out our biophysical analysis capabilities by
My work was split between formulation development for T-cell therapy products and data science including database development and management. In this role I
As a scientist in Drug Product, my core responsibilities are to
As the Lead Biophysical Scientist in Drug Product, I
As a member of the High-Throughput and Automation work-stream, I
During my tenure in Drug Product, I became interested in visual design, data science, and programming. To this end I
PIs: David Weis (Weis Research Group Homepage), David Volkin, Russ Middaugh
In my role as an associate researcher, I led research projects focused on using Hydrogen Exchange Mass Spectrometry (HX-MS) for
I also supervised and mentored graduate students whose projects involved HX-MS.
PIs: David Volkin, Russ Middaugh
My job as a postdoctoral researcher was to design and execute study packages purchased by pharmaceutical corporations outsourcing their development; these studies included excipient screens, formulation development packages, and characterization packages.
As the center’s expert in analytical ultracentrifugation, I was responsible for executing that portion of all work packages.
PI: Thomas Laue
My graduate thesis work centered around using analytical ultracentrifugation to
Biophysical Analysis
Mass Spectrometry
Liquid Handling Robotics
Chemical and Charge Analysis
Particle Analysis
Chromatography
Data Analysis
Data Science and Programming
Conference Presentations
A High-Throughput, Comprehensive Degradation Temperature Panel is Vital for AAV Development (watch here)
The Critical Role of First-Principles, Fundamental Techniques in Gene Therapy (watch here)
Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) as an Important Orthogonal Tool for Gene Therapy Characterization (watch here)
First Author Publications
Evaluation of Hydrogen Exchange Mass Spectrometry as a Stability-Indicating Method for Formulation Excipient Screening for an IgG4 Monoclonal Antibody. J Pharm Sci. (2018), DOI.
Empirical Correction for Differences in Chemical Exchange Rates in Hydrogen Exchange-Mass Spectrometry Measurements. Analytical Chemistry (2017), DOI.
High-Definition Mapping of Four Spatially Distinct Neutralizing Epitope Clusters on RiVax, a Candidate Ricin Toxin Subunit Vaccine. Clin Vaccine Immunol. (2017), DOI.
Nonideality in high concentration solutions. University of New Hampshire Doctoral Dissertations (2012), LINK.
Publications from Mentored Students
Characterization of Excipient Effects on Reversible Self-association, Backbone Flexibility, and Solution Properties of an IgG1 Monoclonal Antibody at High Concentrations: Part 2. J Pharm Sci. (2019), DOI.
Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry. J Pharm Sci. (2018), DOI.
A Collection of Single-Domain Antibodies that Crowd Ricin Toxin’s Active Site. Antibodies (2018), DOI.
Collaborative Publications
Recombinant Subunit Rotavirus Trivalent Vaccine Candidate: Physicochemical Comparisons and Stability Evaluations of Three Protein Antigens. J Pharm Sci. (2020), DOI.
Fine-Specificity Epitope Analysis Identifies Contact Points on Ricin Toxin Recognized by Protective Monoclonal Antibodies. Immunohorizons (2018), DOI.
Structural Characterization and Physicochemical Stability Profile of a Double Mutant Heat Labile Toxin Protein Based Adjuvant. J Pharm Sci. (2017), DOI.
Using homology modeling to interrogate binding affinity in neutralization of ricin toxin by a family of single domain antibodies. Proteins (2017), DOI.
Effects of Protein Conformation, Apparent Solubility, and Protein-Protein Interactions on the Rates and Mechanisms of Aggregation for an IgG1Monoclonal Antibody. J Phys Chem B. (2016), DOI.
Challenges and opportunities of using liquid chromatography and mass spectrometry methods to develop complex vaccine antigens as pharmaceutical dosage forms. J Chromatogr B Analyt Technol Biomed Life Sci. (2016), DOI.
Spa47 is an oligomerization-activated type three secretion system (T3SS) ATPase from Shigella flexneri. Protein Sci. (2016), DOI.
Site-Specific Hydrolysis Reaction C-Terminal of Methionine in Met-His during Metal-Catalyzed Oxidation of IgG-1. Mol Pharm. (2016), DOI.
Biophysical Characterization of the Type III Secretion System Translocator Proteins and the Translocator Proteins Attached to Bacterium-Like Particles. J Pharm Sci. (2015), DOI.
A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation. PLoS One (2015), DOI.
Impact of detergent on biophysical properties and immune response of the IpaDB fusion protein, a candidate subunit vaccine against Shigella species. Infect Immun. (2015), DOI.
N-terminus of IpaB provides a potential anchor to the Shigella type III secretion system tip complex protein IpaD. Biochemistry (2013), DOI.