Recombinant Subunit Rotavirus Trivalent Vaccine Candidate: Physicochemical Comparisons and Stability Evaluations of Three Protein Antigens
Although live attenuated Rotavirus (RV) vaccines are available globally to provide protection against enteric RV disease, efficacy is …
Characterization of Excipient Effects on Reversible Self-Association, Backbone Flexibility, and Solution Properties of an IgG1 Monoclonal Antibody at High Concentrations: Part 2
In this work, we continue to examine excipient effects on the reversible self-association (RSA) of 2 different IgG1 monoclonal …
Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry (HX-MS)
We have utilized hydrogen exchange-mass spectrometry (HX-MS) to characterize local backbone flexibility of four well-defined IgG1-Fc …
Fine-Specificity Epitope Analysis Identifies Contact Points on Ricin Toxin Recognized by Protective Monoclonal Antibodies
Ricin is a fast-acting protein toxin classified by the Centers for Disease Control and Prevention as a biothreat agent. In this report, …
Evaluation of Hydrogen Exchange Mass Spectrometry as a Stability-Indicating Method for Formulation Excipient Screening for an IgG4 Monoclonal Antibody
Antibodies are molecules that exhibit diverse conformational changes on different timescales, and there is ongoing interest to better …
High-Definition Mapping of Four Spatially Distinct Neutralizing Epitope Clusters on RiVax, a Candidate Ricin Toxin Subunit Vaccine
RiVax is a promising recombinant ricin toxin A subunit (RTA) vaccine antigen that has been shown to be safe and immunogenic in humans …
Structural Characterization and Physicochemical Stability Profile of a Double Mutant Heat Labile Toxin Protein Based Adjuvant
A novel protein adjuvant double-mutant Escherichia coli heat-labile toxin, LT (R192G/L211A) or dmLT, is in preclinical and early …
Empirical Correction for Differences in Chemical Exchange Rates in Hydrogen Exchange-Mass Spectrometry Measurements
A barrier to the use of hydrogen exchange-mass spectrometry (HX-MS) in many contexts, especially analytical characterization of various …
Using Homology Modeling to Interrogate Binding Affinity in Neutralization of Ricin Toxin by a Family of Single Domain Antibodies
In this report we investigated, within a group of closely related single domain camelid antibodies (VHHs), the relationship between …
Challenges and opportunities of using liquid chromatography and mass spectrometry methods to develop complex vaccine antigens as pharmaceutical dosage forms
Liquid chromatographic methods, combined with mass spectrometry, offer exciting and important opportunities to better characterize …
Effects of Protein Conformation, Apparent Solubility, and Protein-Protein Interactions on the Rates and Mechanisms of Aggregation for an IgG1Monoclonal Antibody
Non-native protein aggregation is a key degradation pathway of immunoglobulins. In this work, the aggregation kinetics of an …
Site-Specific Hydrolysis Reaction C-Terminal of Methionine in Met-His during Metal-Catalyzed Oxidation of IgG-1
The metal-catalyzed oxidation by Fe(II)(EDTA)/H2O2 of IgG-1 leads to the site-specific hydrolysis of peptide bonds in the Fc region. …
Spa47 is an oligomerization‐activated type three secretion system (T3SS) ATPase from Shigella flexneri
Gram‐negative pathogens often use conserved type three secretion systems (T3SS) for virulence. The Shigella type three secretion …
Biophysical Characterization of the Type III Secretion System Translocator Proteins and the Translocator Proteins Attached to Bacterium-Like Particles
Diarrhea caused by Shigella, Salmonella, and Yersinia is an important public health problem, but development of safe and effective …
A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation
Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant …
Impact of Detergent on Biophysical Properties and Immune Response of the IpaDB Fusion Protein, a Candidate Subunit Vaccine against Shigella Species
Shigella spp. are causative agents of bacillary dysentery, a human illness with high global morbidity levels, particularly among …
The N-terminus of IpaB provides a potential anchor to the Shigella type III secretion system tip complex protein IpaD
The type III secretion system (T3SS) is an essential virulence factor for Shigella flexneri, providing a conduit through which …
Nonideality in High Concentration Solutions
Our current understanding of molecular interactions, the kinetics, equilibria, and thermodynamics of biochemical reactions, is based …