Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) is almost universally used and revered as a gold-standard technique, particularly for AAV empty/full; it is also almost universally maligned as an outdated, cumbersome, and low-throughput technique - with most of these views based on previous hardware and software implementations and thus outmoded. This talk seeks to introduce the audience to the modern AUC platform with case studies covering the characterization of AAVs and DNA drug substance. Outmoded myths regarding AUC are dispelled, showing that SV-AUC can be a medium-throughput, validated tool delivering first-principle multiattribute insights in one method - all in the first run with no prior knowledge of the product.